T3.2 Clear Optically Matched Panoramic Access Channel Technique (COMPACT) for Large Volume Deep Brain Imaging
Meng Cui (Purdue University)
With the advance of function molecular indicators and actuators, neurophotonics has become a powerful paradigm for discovering the principle of brain function. However, a major limitation of employing light to study mammalian brain is the limited access depth. Even with the advance of multiphoton imaging, the majority of implementation in mammalian brain is limited to ~ 1 mm depth, about the thickness of mouse neocortex. The majority of mouse brain remains inaccessible, not to mention the brain of larger mammals. To investigate deeper brain regions, invasive miniature optical probes are often employed. A key difficulty with the available optical probes is the tiny tissue access volume, which is only a small fraction of the inserted probe volume. The design of miniature optical probes has largely remained the same over the past two decades. Can we fundamentally change the design of miniature optical probes and greatly increase the tissue access volume? Here we report our latest technology development, Clear Optically Matched Panoramic Access Channel Technique (COMPACT), which can effectively increase the tissue access volume by ~ three orders of magnitude. The development of COMPACT will provide tremendous capacity, flexibility and adaptability to the neuroscience research. We will discuss our experiment on employing COMPACT for two-photon calcium imaging of deep mammalian brain.
Event Timeslots (1)
SYMPOSIUM T3 – OPTICAL DEVICE AND PHENOMENON
-
Meng Cui
Location: Technological Institute M128